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Normally, the anterior pituitary secretes thyroid-stimulating hormone (TSH) in response to hypothalamic thyrotropin-releasing hormone (TRH), stimulating the thyroid to release approximately 20% triiodothyronine (T3) and 80% thyroxine (T4) (Fig
For best results, you might allow the vial to sit at room temperature for a few minutes before reconstitution to avoid a thermal shock to the lyophilized powder, but the water itself will work just as effectively.
Orthopaedic Journal of Sports Medicine (2025) 2